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DC Field | Value | Language |
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dc.rights.license | http://creativecommons.org/licenses/by-nc-nd/4.0 | es_MX |
dc.contributor.author | Salvador Hurtado De la Peña | es_MX |
dc.creator | ALEJANDRO ANGELES ESPINO | es_MX |
dc.date.accessioned | 2020-05-01T02:25:54Z | - |
dc.date.available | 2020-05-01T02:25:54Z | - |
dc.date.issued | 2016-12-16 | - |
dc.identifier.uri | http://repositorio.ugto.mx/handle/20.500.12059/1763 | - |
dc.description.abstract | El cultivo del agave y el tequila tienen importancia económica y social por los empleos en campo y la industria, además por las divisas de la exportación. El tizón foliar causado por Cercospora agavicola representa un alto riesgo en la zona de Los Altos de Jalisco, debido a que el clima favorece la dispersión y germinación de las esporas. Se inocularon plántulas obtenidas a partir de yemas axilares, mismas que se irradiaron con rayos gamma Co60 a 0 Gy (testigo), 5 Gy, 10 Gy, 15 Gy, 20 Gy, 25 Gy y 30 Gy, conformándose un experimento con siete tratamientos y cinco repeticiones. Muestras de síntomas de plantas con tizón se colectaron en campo. El hongo se aisló, purificó y multiplicó en medio papa dextrosa agar y agave (PDAA) a 25 °C. Los foliolos se colocaron en cajas Petri con papel secante humedecido. La inoculación se efectuó a una concentración de 20 000 esporas/mL–1. La presencia de lesiones se evaluó en mm2 a los 21 días. El tamaño de la lesión disminuyó conforme la dosis de radiación se incrementó; se clasificaron en tres grupos de acuerdo con el grado de la lesión: 0 Gy y 5 Gy mayor al 90%, 10 Gy y 15 Gy de 60% a 80% y 20 Gy, 25 Gy y 30 Gy menor al 20%. Las pruebas de patogenicidad deben corroborarse en plantas de seis meses con hojas engrosadas y endurecidas | es_MX |
dc.language.iso | spa | es_MX |
dc.publisher | Universidad de Guanajuato | es_MX |
dc.relation | https://doi.org/10.15174/au.2016.1121 | - |
dc.rights | info:eu-repo/semantics/openAccess | es_MX |
dc.source | Acta Universitaria: Multidisciplinary Scientific Journal. Vol. 26 No. 6 (2016) | es_MX |
dc.title | Evaluación no destructiva de la patogenicidad de Cercospora agavicola en vitroplántulas de agave azul tequilero irradiadas con rayos gamma Co60 | es_MX |
dc.title.alternative | Nondestructive evaluation of pathogenicity of Cercospora agavicola on agave azul tequilero plantlets irradiated with gamma rays Co60 | - |
dc.type | info:eu-repo/semantics/article | es_MX |
dc.creator.id | info:eu-repo/dai/mx/cvu/145026 | es_MX |
dc.subject.cti | info:eu-repo/classification/cti/6 | es_MX |
dc.subject.keywords | Agave | es_MX |
dc.subject.keywords | Cercospora agavicola | es_MX |
dc.subject.keywords | Mutagénesis | es_MX |
dc.subject.keywords | Rayos gamma | es_MX |
dc.subject.keywords | Agave | - |
dc.subject.keywords | Cercospora agavicola | - |
dc.subject.keywords | Gamma rays | - |
dc.subject.keywords | Mutagenic | - |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_MX |
dc.creator.two | GIL VIRGEN CALLEROS | es_MX |
dc.creator.three | ALBERTO JULIAN VALENCIA BOTIN | es_MX |
dc.creator.four | CARLOS RAMIREZ SERRANO | es_MX |
dc.creator.five | LYDIA CONCEPCION PAREDES GUTIERREZ | es_MX |
dc.creator.idtwo | info:eu-repo/dai/mx/cvu/74671 | es_MX |
dc.creator.idthree | info:eu-repo/dai/mx/cvu/38850 | es_MX |
dc.creator.idfour | info:eu-repo/dai/mx/cvu/121053 | - |
dc.creator.idfive | info:eu-repo/dai/mx/cvu/200253 | - |
dc.description.abstractEnglish | Agave crop and tequila industry have cultural, economic and social impact do to employ-ment offered under Agro and industrial fields. Besides, currencies obtained by exportation. Fungal agave disease caused by C. agavicola represents high risk in the Altos zone in Jalisco, since environmental conditions favour incidence and severity of disease. Inocula-tion was carried out on plantlets obtained from axillary buds culture and irradiated by gamma rays Co60: 0 Gy, 5 Gy, 10 Gy, 15 Gy, 20 Gy, 25 and 30 Gy. Constituting and ex-periment involving 7 treatments with 5 replicates each. Samples were collected from plants showing blight symptoms in the field. Fungus was isolated, grown and purified onto PDAA (for its acronym in spanish) medium at 25 °C. Subsecuently, fungus was inoculated directly onto leafs of Agave plantlets in Petri dishes containing moist filter paper in a suspension solution of 20 000 spores/mL–1; all material was transferred onto sterile Petri dishes. Size (mm2) of lesions was evaluated 21 days later. Lesion size decrease as the irradiation dose increased. Lesions were classified into 3 groups: 0 Gy and 5 Gy greater than 90% of leaf area affected, 10 Gy and 15 Gy between 60% and 80% of leaf area affected and 20 Gy, 25 Gy and 30 Gy less 20% of leaf area affected. Pathogenicity tests should be corroborated on 6 months old plants with hardened and thick leaves | - |
Appears in Collections: | Revista Acta Universitaria |
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File | Description | Size | Format | |
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Evaluación no destructiva de la patogenicidad de Cercospora agavicola en vitroplántulas de agave azul tequilero irradiadas con rayos gamma Co60.pdf | 757.26 kB | Adobe PDF | View/Open |
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